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Platelet activation analysis by Flow cytometry After inhibition of in vitro platelet activation with prostagladin E1, 5 L of the samples were stained with 1 l of a fluorescein isothiocyanate (FITC) conjugated monoclonal antibody against GPIIbIIIa (CD41a, BD Biosciences CA), 1 l of a phycoerythrin (PE)-conjugated monoclonal antibody against P-selectin (CD62P, BD Biosciences, CA) and 1 l of a allophycocyanin (APC)-conjugated monoclonal antibody against glycophorin A (GPA, Dakopatts Glostrup, Denmark)
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