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The MTR gene works in the final step to regenerate homocysteine into methionine using methyl-B12 (methylcobalamin) MTRR regenerates the methylcobalamin for MTR to use again.[ref] Both are a vital part of the methylation cycle

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H 2 S is mainly generated by three endogenous enzymes with different organ/tissue distributions, all of which are related to cysteine metabolism: the trans-sulphuration pathway pyridoxal-5-phosphate-dependent-CBS and CSE, and 3-MST