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Hierarchical clustering was obtained through filtering procedures based on technical replicates coefficient of variation (CV%), retention time, and mass accuracy, excluding analytes eluting close to the solvent front, with poor response for coefficient of separation k , peak shape, and spectrum purity in both full scan and data-dependent mode

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The epigenome drifts significantly with age, silencing genes associated with repair and activating genes associated with inflammation and dysfunction

The lyophilization process creates slightly different textures each time