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HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)

Conclusion and recommendations In general, our study showed a higher mean as well as proportion of uric acid with the use of a DTG-based regimen, whereas a higher proportion of hsCRP levels was found among those treated by ATV/r-based regimens