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The concentration of complementary deoxyribonucleic acid (cDNA) after reverse transcription were also determined using NanoDrop Spectrophotometer

In Vitro Skin Irritation Test HaCaT keratinocytes were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin/streptomycin and were maintained under 37 C with humidified 5% CO 2