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Additional information Peer review information Nature Chemical Biology thanks Dohoon Kim and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

Factors Influencing Bacteriostatic Water Volume The volume of bacteriostatic water needed depends on several factors: Peptide Quantity: Peptides are typically supplied in vials containing a specific amount, such as 2 mg, 5 mg, or 10 mg