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The rubber stopper is exposed to the open air, and failing to sanitize it is the most common way to introduce bacteria into your sterile solution.

Specific Dosage Guidelines Severe Deficiency : For patients with severe vitamin B12 deficiency, an initial dose of 1 mg (1000 g) of intramuscular hydroxocobalamin is commonly administered

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On a 0.25mg dose, that is a 40% error