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Signature discovery approach for sample matching of a nerve-agent precursor using liquid chromatography-mass spectrometry, XCMS, and chemometrics
GLP-1 is rapidly cleaved by the widely expressed dipeptidyl-peptidase-IV (DPP-IV), which removes two N-terminal amino acids, resulting in GLP-1(9-36) with little glucoregulatory properties but with potential cardiovascular effects [18]
(By the way, this is precisely what makes HILIC an orthogonal technique to RPLC!) By contrast, retention in many HILIC columns depends on the semi-immobilized water layer formed at the interface of the polar stationary phase and the organic-rich mobile phase
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