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The next day, 2 ml of each of the cultures was added to 100 ml LB liquid medium containing 100 mg/L of ampicillin and allowed to grow at 37 until the optical density at 600 nm (OD 600 ) reaches between 0.4 and 0.6

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The proposed net effect in experimental systems is a shift in energy substrate utilisation without the hyperglycaemic liability that pure glucagon agonism would carry alone

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