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Signs that warrant evaluation include: pain radiating down the arm or leg persistent numbness muscle weakness worsening symptoms over time Imaging and a physical examination help determine the underlying cause of the problem

Purification and Detection High performance liquid chromatography (HPLC) purification: The synthesized polypeptides need to be purified by HPLC to remove impurities and unreacted raw materials, ensuring that the purity meets the pharmaceutic-grade standard

[2] One of eight B vitamins, it serves as a vital cofactor in DNA synthesis and both fatty acid and amino acid metabolism

Science 312 , 734737 (2006)