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This may reflect improved GLP-1 sensitivity in R, potentially contributing to improved -cell function by its improved insulinotropic effect that has been shown to be more pronounced during normoglycemic conditions 57

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Agonist dissociation was monitored immediately after washing in 10 M exendin(9-39) by TR-FRET at 37 C, with 50 s delay and 300 s integration time, and binding quantified as 520 nm over 620 nm signal after nonspecific binding subtraction with excess (10 M) unlabeled agonist