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Cell lysates were subjected to Western blot analysis for detecting CD68 (a and b) or LAMP1 (a and c) levels
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Quality filtered 16S rRNA sequences were clustered into operational taxonomic units (OTUs), with 97% similarity, by closed reference OTU-picking using the UCLUST algorithm and GreenGenes reference database (v13.5) as implemented in Quantitative Insights into Microbial Ecology (QIIME versions 1.6 and 1.7) [64]
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