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The cell lysate was diluted to 0.8 mg protein/ml with dilution buffer (10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1x Protease inhibitors), and 1 mg protein was used per replicate
FDA approval was based on the SOUL cardiovascular outcomes trial (CVOT) that demonstrated a 14% relative reduction in risk of MACE in patients treated with Rybelsus 14 mg orally once daily for approximately 5 years compared to those who received placebo
Record the concentration immediately after reconstitution
Retinol, -carotene, and -tocopherol were eluted using 0.1% formic acid in methanol as solvent at a flow rate of 0.600 ml/min
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