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Huh7 and Hep3B cells were cultured in high-glucose Dulbeccos modified Eagles medium (DMEM, Huh7) and minimum essential medium (MEM, Hep3B), containing 10% fetal bovine serum (FBS) in a 37 C incubator with 5% CO 2
28 The DAG was built by consensus of the research team with variables that were considered clinically relevant based on biological plausibility or evidence from the literature
"Pivalate-generating prodrugs and carnitine homeostasis." Pharmacol Rev 2002;54:589-98
This ultimately leads to steatosis, inflammatory infiltration, and hepatocyte apoptosis [366]
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