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Low levels of B12 can cause a variety of serious side effects, including: Weakness and fatigue Lightheadedness Memory loss Loss of balance Numbness or tingling in the arms and legs Muscle weakness Shakiness Changes in mood, including depression Low blood pressure Vision problems Bladder problems Twitching of the eye Stabbing pain in the palms of the hands Pale skin In severe cases, vitamin B12 deficiency can result in anemia, a serious condition where the body cannot make enough red blood cells
Avoid repeated freeze-thaw cycles, which reduce recovery of the 43-residue thymosin beta 4 chain in particular, label every aliquot with the reconstitution date, and handle throughout under sterile laboratory conditions
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