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Immunohistochemistry for islet histology For islet histology, paraffin-embedded 5 m slices were stained for insulin (ab195956, 1:1500, Alexa Fluor 555-conjugated secondary antibody: ab150186, 1:500, Abcam) and glucagon (ab92517, 1:2000, Alexa Fluor 488-conjugated secondary antibody ab150077, 1:1000, Abcam)
The importance of GLP-1 is demonstrated in experiments showing that it accounts for up to 60% of postprandial insulin secretion in healthy individuals
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