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The slides were heated for 30 min at 60 C followed by antigen retrieval using a steamer, then processed by the RNAscope Multiplex Fluorescent Reagent kit v2 (Advanced Cell Diagnostics, 323270) according to the manufacturers instructions
Step 2: Address the Liver
The field-level measurements for each individual rat were averaged to yield a single data point per rat, meaning the individual animal remained the true statistical unit in all downstream analyses
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