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Full-length thymosin beta-4 contains 43 amino acids and has a molecular mass close to 4.9 kDa

The adjacent acidic residues may influence how the molecule behaves in aqueous systems and how it interacts with charged molecular surfaces

thus, decreased levels can make them more susceptible to damage from oxidative stress, leading to rupture and causing hyperbilirubinemia and anemia ( GSSD can be diagnosed based on clinical presentation, an elevated urinary 5-oxoprolinuria concentration, and low GSS activity in erythrocytes or fibroblasts

Cheng B, Zhu J, Yang T et al (2020) Vitamin A deficiency exacerbates autism-like behaviors and abnormalities of the enteric nervous system in a valproic acid-induced rat model of autism