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Peroxynitrite (ONOO - ,0.1 M stock solution in 0.3 M NaOH-NaCl) was diluted to less than 1/100 with ice-cold water, just prior to quick exposure to HGF in PBS by vortexing at 1:2000 (see Figure 1a for the experimental design) 32
Likewise, no effect was observed on downstream components of the Insulin/IGF-1 signaling pathway with mAb treatment in females (Supplementary Fig 3), but L2-Cmu attenuated S6 activation in male hypothalamus (Supplementary Fig 4c
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