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Rat'ko AA et al

Clinical trials have consistently demonstrated that this restoration leads to a dramatic, measurable reduction in specific biomarkers of systemic oxidative damage, such as TBARS (thiobarbituric acid reactive substances) and F2-isoprostanes, which are direct indicators of lipid membrane destruction

33 Consistent with this concept, in vivo zebrafish injury models have demonstrated that lipid peroxidation can act as a spatial redox relay over hundreds of micrometers, providing a biologically relevant framework for how oxidative signals may propagate across multicellular tissues

That single-use window makes sterile water unsuitable for multi-day reconstitution protocols