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GSH depletion also activates the nuclear factor erythroid 2-related factor 2 ( NRF2 ), a transcription factor that induces expression of several cellular antioxidant defense genes 16,17
doi:10.1001/jamanetworkopen.2025.2281
Most common triggers include large meals, high fat foods, and eating past fullness
Researchers favor this approach for its accessibility, well-documented protocols, and compatibility with diverse biological sample types including cell lysates and tissue homogenates
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