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A low acid content can also cause poor vitamin absorption
In vitro cultures Following 5-6 h of fertilization, oocytes were washed twice in Charles Rosenkrans (CR1aa) culture medium, containing 0.4 mM sodium pyruvate, 5 mM lactate, 5% calf serum, 20 g/mL streptomycin sulphate
Ebanks JP, Wickett RR, Boissy RE
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