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Cells were reduced with 10 mM DTT or oxidized with 5 mM diamide for 5 min prior to treatment with 25 mM NEM for 10 min before lysis
Independent samples t -tests Results from independent samples t -tests on the nine unadjusted metabolite concentrations (glutathione, glutamate, and glutamine in each of the three voxels) are given in Table 1