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After 18 h incubation at 27C, excess media was removed from the wells and replaced with 100 L of solution C containing either 9 10 3 (1 MOI) or 2.7 10 4 (3 MOI) zoospores per well for 1 hr, after which the inoculation solution as removed and replaced with 100 L solution A (25, 28)
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It occurred only once in about 200 analysed sequences
Post-Treatment: Most clients leave feeling refreshed and revitalized, with minimal downtime
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