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Consider the tick-borne Lyme disease, caused by the Borrelia burgdorferi bacteria, which sometimes manifests itself in bullseye pattern skin lesions, but can later progress to muscle dysfunction, arthritis, facial palsy, and more

It should be stored at room temperature and not frozen, and should be used within 28 days of first puncture

The math is straightforward: Total Peptide: 100mg Desired Concentration: 10mg/mL Calculation: (Total Peptide) / (Desired Concentration) = Solvent Volume Result: 100mg / 10mg/mL = 10mL of BAC water Adding 10mL of BAC water to your 100mg vial of GHK-Cu will give you a final solution where every 1mL of liquid contains exactly 10mg of the peptide

2b) and therefore we used cell size normalization in the quantification of lysosome positioning (see Methods section)