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Copper can raise an enzyme called MMP-1, and MMP-1 breaks collagen down
To purify the enzyme, the frozen stock was warmed to room temperature to lyse the cells ( E.coli BL21(DE3) strain Rosetta expresses lysozyme that facilitates cell lysis upon thawing after a freeze-thaw cycle)
Mitochondrial Vulnerability: Mitochondria cannot synthesise glutathione and depend entirely on membrane transporters for supply
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