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HEK293 C24 cells stably expressing the H188 FRET reporter were obtained by G418 selection and grown in monolayers to 70% confluency in 100 cm 2 tissue culture dishes and were then transfected with plasmids (11 g/dish) encoding human GLP-1R, human Y2-R, or human Y1-R
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I used to constantly feel like I needed to eat, and now I literally just put down the food and walk away when Im satisfied
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