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K-means clustering of amplicon sequence variant data confirmed that 5-Amino-1MQ treatment generated a distinct cecal microbiome profile from all other treatment groups including diet-switched vehicle controls indicating that NNMT inhibition produces microbiome changes that are separable from those produced by dietary composition alone

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The result is reported in EU/mg on the Certificate of Analysis at cell-culture-reference acceptance limits, giving researchers an upfront figure when planning sterile cell-based assays and minimizing the risk of confounding cytokine responses