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pSSBR was then treated with 10 units of Nb.BtsI (NEB) at 37 C for 4 h, generating a plasmid with single-strand breaks (nicks) on opposing strands, separated by a distance of 36 bp
The samples were centrifuged using TOMY Flexin Bench Top centrifuge at 350 rpm/5 minutes, aliquoted in micro centrifuge tubes, and then stored at 80C
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Discovery and history Discovered in 1973 by Dr
Low levels of B12 can cause fatigue, nervousness, dizziness, numbness, and tingling in the fingers and toes
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