After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions
Helps even out skin tone and reduce tanning
Several technical reasons may explain our distinctive findings, including differences in the microbiome sampling site, the limited n per group, singly housing mice, or the use of highly similar lean, Western, and high-fat diets which systematically exchanged fat content with carbohydrate content
The instrument was calibrated using 0, 5, 10, 50, 100 and 500 ppb of certified multi-element ICP-MS standard calibration solutions (ICP-MS-CAL2-1, ICP-MS-CAL-3 and ICP-MS-CAL-4, Accustandard) for a range of elements